Description
This package can be used as part of the NGS Library Preparation protocol for HaloPlex HS Target Enrichment System. This package contains:
- Two sets of barcode indices: Agilent HaloPlex HS yellow and Agilent HaloPlex HS orange.
- Each barcode index has:
- A name
- A sequence
- A type: I5, I7, or either
- This is based on document: HaloPlex HS Target Enrichment System For Illumina Sequencing. Protocol. Version C1, December 2016, https://www.agilent.com/cs/library/usermanuals/public/G9931-90000.pdf
- Each barcode index has:
- Two index plate designs: Agilent HaloPlex HS yellow and Agilent HaloPlex HS orange. Index layout with 8×12 indices.
This package works in combination with the MiSeq package from SLIMS.
How to use the package
After you have imported the package, your SLIMS instance will have:
- Two new index sets in the DNA Index Set module with all the barcode indices.
- Two new plate designs, that have matching for positions on a 96 well plate and a barcode index.
Pre-requisite
The MiSeq package from Package Browser module should be installed first.
Configuration
The imported index sets are based on official index documentation, so no update is necessary.
If needed, it is possible to rename the index sets and the indices. The instrument type on the DNA Index set can also be updated.
It is also possible to modify or add index plate designs.
The necessary workflows should be configured and the matching Sequencing Settings should be created.
Where to Look Next
These references have further information on how to configure or use the package contents after the initial installation and integration.
- SLIMS Administration Manual:
- Workflows > SLIMS NGS