Description
This package can be used as part of the NGS Library Preparation protocol for HaloPlex Target Enrichment System. This package contains:
- One set of barcode indices: Agilent HaloPlex.
- Each barcode index has:
- A name
- A sequence
- A type: I5, I7, or either
- This is based on document: HaloPlex Target Enrichment System For Illumina Sequencing. Protocol. Version G0, July 2018., https://www.agilent.com/cs/library/usermanuals/public/G9900-90001_HaloPlex_Target_Enrichment%20_Manual.pdf
- Each barcode index has:
- One index plate design: Agilent HaloPlex. Index layout with 8×12 indices.
This package works in combination with the MiSeq package from SLIMS.
How to use the package
After you have imported the package, your SLIMS instance will have:
- One new index set in the DNA Index Set module with all the barcode indices.
- One new plate design, that has matching for positions on a 96 well plate and a barcode index.
Pre-requisite
The MiSeq package from Package Browser module should be installed first.
Configuration
The imported index sets are based on official index documentation, so no update is necessary.
If needed, it is possible to rename the index sets and the indices. The instrument type on the DNA Index set can also be updated.
It is also possible to modify or add index plate designs.
The necessary workflows should be configured and the matching Sequencing settings should be created.
Where to Look Next
These references have further information on how to configure or use the package contents after the initial installation and integration.
- SLIMS Administration Manual:
- Workflows > SLIMS NGS
