Description
This package can be used as part of the NGS Library Preparation protocol for SureSelect XT HS2 DNA System. This package contains:
- Four sets of barcode indices: Agilent SureSelect XT HS2 green, Agilent SureSelect XT HS2 red, Agilent SureSelect XT HS2 orange, and Agilent SureSelect XT HS2 blue.
- Each barcode index has:
- A name
- A sequence
- A type: I5, I7, or either
- This is based on document: SureSelect XT HS2 DNA System DNA Library Preparation and Target Enrichment for the Illumina Platform. Protocol. Version D0, April 2021, https://www.agilent.com/cs/library/usermanuals/public/G9983-90000.pdf
- Each barcode index has:
- Four index plate designs: Agilent SureSelect XT HS2 green, Agilent SureSelect XT HS2 red, Agilent SureSelect XT HS2 orange, and Agilent SureSelect XT HS2 blue. Index layout with 8×12 indices.
This package works in combination with the MiSeq package from SLIMS.
How to use the package
After you have imported the package, your SLIMS instance will have:
- Four new index sets in the DNA Index Set module with all the barcode indices.
- Four new plate designs, that have matching for positions on a 96 well plate and a barcode index.
Pre-requisite
The MiSeq package from Package Browser module should be installed first.
Configuration
The imported index sets are based on official index documentation, so no update is necessary.
If needed, it is possible to rename the index sets and the indices. The instrument type on the DNA Index set can also be updated.
It is also possible to modify or add index plate designs.
The necessary workflows should be configured and the matching Sequencing settings should be created.
Where to Look Next
These references have further information on how to configure or use the package contents after the initial installation and integration.
- SLIMS Administration Manual:
- Workflows > SLIMS NGS