Agilent SureSelect XT Index Set

Description

This package can be used as part of the NGS Library Preparation protocol for SureSelect^XT Automated Target Enrichment for the Illumina Platform. This package contains:

• One set of barcode indices: Agilent SureSelect XT.
  • Each barcode index has:
    • A name
    • A sequence
    • A type: I5, I7, or either
  • This is based on document: SureSelect^XT Automated Target Enrichment for the Illumina Platform Automated using Agilent NGS Workstation Option B. Protocol. Version N0, November 2020,https://www.agilent.com/cs/library/usermanuals/public/G7550-90000.pdf
• One index plate design: Agilent SureSelect XT. Index layout with 8×12 indices.

This package works in combination with the MiSeq package from SLIMS.

How to use the package

After you have imported the package, your SLIMS instance will have:

• One new index set in the DNA Index Set module with all the barcode indices.
• One new plate design, that has matching for positions on a 96 well plate and a barcode index.

Pre-requisite

The MiSeq package from Package Browser module should be installed first.

Configuration

The imported index sets are based on official index documentation, so no update is necessary.

If needed, it is possible to rename the index sets and the indices. The instrument type on the DNA Index set can also be updated.

It is also possible to modify or add index plate designs.

The necessary workflows should be configured and the matching Sequencing settings should be created.

Where to Look Next

These references have further information on how to configure or use the package contents after the initial installation and integration.

• SLIMS Administration Manual:
  • Workflows > SLIMS NGS