Agilent SureSelect XT low input Index Set

Description

This package can be used as part of the NGS Library Preparation protocol for SureSelect^XT Low Input Target Enrichment System. This package contains:

• Two sets of barcode indices: Agilent SureSelect XT Low Input Red and Agilent SureSelect XT Low Input Yellow.
  • Each barcode index has:
    • A name
    • A sequence
    • A type: I5, I7, or either
  • This is based on document: SureSelect^XT Low Input Target Enrichment System For Illumina Multiplexed Sequencing Platforms. Protocol. Version E1, April 2021, https://www.agilent.com/cs/library/usermanuals/public/G9703-90000.pdf
• Two index plate designs: Agilent SureSelect XT Low Input Red and Agilent SureSelect XT Low Input Yellow. Index layout with 8×12 indices.

This package works in combination with the MiSeq package from SLIMS.

How to use the package

After you have imported the package, your SLIMS instance will have:

• Two new index sets in the DNA Index Set module with all the barcode indices.
• Two new plate designs, that have matching for positions on a 96 well plate and a barcode index.

Pre-requisite

The MiSeq package from Package Browser module should be installed first.

Configuration

The imported index sets are based on official index documentation, so no update is necessary.

If needed, it is possible to rename the index sets and the indices. The instrument type on the DNA Index set can also be updated.

It is also possible to modify or add index plate designs.

The necessary workflows should be configured and the matching Sequencing settings should be created.

Where to Look Next

These references have further information on how to configure or use the package contents after the initial installation and integration.

• SLIMS Administration Manual:
  • Workflows > SLIMS NGS