10x Genomics Chromium Single Cell Index Set

Description

This package can be used as part of the NGS Library Preparation protocol for Chromium i7 Multiplex Kit – v2,v3,v3.1. This package contains:

One set of barcode indices: Chromium i7 Multiplex Kit – v2,v3,v3.1.
- Each barcode index has:
  - A name
  - A sequence
  - A type: I5, I7, or either
- This is based on the document linked here: https://support.10xgenomics.com/single-cell-gene-expression/sequencing/doc/specifications-sample-index-sets-for-single-cell-3 for v2, v3 and v3.1.
One index plate design: Chromium i7 Multiplex Kit – v2,v3,v3.1 which has an Index layout with 8×12 indices.

This package works in combination with the MiSeq package from SLIMS.

After you have imported the package, your SLIMS instance will have:

One new index set in the DNA Index Set module with all the barcode indices.
One new plate design that has matching for positions on a 96 well plate and a barcode index.

The MiSeq package from the Package Browser module should be installed first.

The imported index sets are based on official index documentation, so no update is necessary.

If needed, it is possible to rename the index sets and the indices. The instrument type on the DNA Index set can also be updated.

It is also possible to modify or add index plate designs.

The necessary workflows should be configured and the matching Sequencing settings should be created.

These references have further information on how to configure or use the package contents after the initial installation and integration.