Description

This package can be used as part of the NGS Library Preparation protocol for Illumina TruSight Amplicon. This package contains:

  • One set of barcode indices: Illumina TruSight Amplicon.
    • Each barcode index has:
      • A name
      • A sequence
      • A type: I5, I7, or either
    • This is based on document: Illumina Adapter Sequences (1000000002694 v16), Illumina Adapter Sequences v16

This package works in combination with the MiSeq package from SLIMS.

How to use the package

After you have imported the package, your SLIMS instance will have:

  • One new index set in the DNA Index Set module with all the barcode indices.

Pre-requisite

The MiSeq package from the Package Browser module should be installed first.

Configuration

The imported index sets are based on official index documentation, so no update is necessary.

If needed, it is possible to rename the index sets and the indices. The instrument type on the DNA Index set can also be updated.

It is also possible to modify or add index plate designs.

This package does not contain a plate design and therefore cannot be used with the default Library preparation step from SLIMS without additional configuration. Create a plate design that best matches the way you assign the indices to your samples in order to use this step.

The necessary workflows should be configured, and also the matching Sequencing settings if needed.

Where to Look Next

These references have further information on how to configure or use the package contents after the initial installation and integration.

  • SLIMS Administration Manual:
    • Workflows > SLIMS NGS